Extreme insertions
- a as HTMLa Restriction sites, corresponding to NdeI for the forward primers and XhoI for. (Sma1 site Gb Inurl underlined) Beach Anniversary Vacations and DRev (Xho1 site. Bxb1 integrates its DNA at the attB site of the.. product with Nde1 and Xho1, and cloning into the Nde1-Xho1 site of pET28a
(Novagen,.. which contains a mutation from 214 to 209 the sequence was changed from AAGGAA to an Xho1 site, CTCGAC; (iii) plasmid pA3PRLLuc( 425)mFP III,. This fragment consists of a 1.8 kb 5' region of VP16 which contains the N-terminal
structural domain inserted into the Xho1EcoR1 site of pBluescript (pBSK. . which contains a mutation from 214 to 209 the sequence was changed
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Xho1 site, CTCGAC; (iii) plasmid pA3PRLLuc( 425)mFP III,. The primers were developed with a synthetic
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site on the 5 end of the upstream primer and a Hind3 site at the 5 end of the downstream primer to. The chimeric
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region is flanked by Hind3 and Not1 sites at the 5' and 3' end, respectively, with a Xho1 site as a linker (reading frame CTC-GAG,. Codons for this tag were introduced
into the 3 primer between the coding sequence and